Reproduction Abstracts

The seminal vesiculitis leads to contamination of the semen with inflammatory cells, causing agglutination, precipitation, decreased sperm motility and increased reactive oxygen species (ROS). This deleterious effect is most pronounced during the cryopreservation process. Therefore, the aim of this study was to evaluate the quality of frozen semen from stallions with seminal vesiculitis after local treatment. Five stallions with seminal vesiculitis were used and local treatmen...

A recent study showed increase of pregnancy rates in donkey frozen semen with addition of seminal plasma post-thaw. The aim of the present study was to evaluate the effect of addition post-thaw of seminal plasma on donkey frozen semen quality. Five ejaculates from three donkeys were used. Semen was diluted (1:1) with a milk-based extender (Botu-Semen). The semen was frozen with Botu-Cryo extender in isothermal box in straws containing 100×106 of total sperm. Th...

One study showed increased fertilization in IVF when added seminal plasma of bulls with high fertility rates in semen of low fertile. The aim of the present study was to evaluate the difference of addition homologous and heterologous seminal on donkey frozen semen. Five ejaculates from two donkeys were used. Semen was diluted (1:1) with a milk-based extender (Botu-Semen). The semen was frozen with Botu-Cryo extender in isothermal box in straws containing 100×106</sup...

There are few studies with donkey semen in the literature. In horses, is related that no difference are in freezing semen using automated systems or Styrofoam box. The aim of the present study was evaluate donkey frozen semen quality in different freezing conditions. Five ejaculates from there donkeys were used. Semen was diluted (1:1) with a milk-based extender. The pellet was resuspended with Botucrio and the straws were packaged. The straws were kept in a refrigerator at 5 ...

The aim of this study was to analyze the effects of refrigeration and cryopreservation on dog sperm special on sperm motility and mitochondrial membrane potential (MMP). A total of 15 ejaculates, first and second fractions, were collected from five dogs. Semen was diluted (80×106 sptz/ml) on Tris-egg-yolk medium with 8% of glycerol (one step), filled into 0.5 ml French straw and refrigerated at 5 °C for 1 h. After straws were suspended 6 cm above liquid ni...

In fixed time artificial insemination (FTAI) is frozen semen is commonly used, however the use of cooled semen is a practice alternative to reduce costs. The aim of this study was compare the difference in DNA fragmentation and plasma membrane integrity between chilled and frozen semen of bulls. Two ejaculates from 20 bulls were collected. Semen was diluted in extender with egg-yolk and glycerol (Botu-Bov™) and divided in two groups: chilled group (CG) samples were cooled...